Comparative evaluation of genotoxicity in tobacco users versus non tobacco users: a pilot study
نویسندگان
چکیده
Background: Tobacco use continues to be prevalent in our society. It contains many substances which are genotoxic in nature. The genotoxicity of these substances can be studied in human peripheral blood lymphocytes by estimating the average number of micronuclei (MN) by cytokinesis block micronuclei assay (CBMN assay). Aims and objectives: To find out whether there is a difference in genotoxicity between tobacco users and non tobacco users. Materials and methods: Five ml of fresh blood was obtained from 15 persons with no habit of tobacco use and 15 persons using tobacco either smoking, chewing or combination of both with clinically normal mucosa in the age group of 20-40 years reporting to the out patient department of PMS College of Dental Sciences & Research, Thiruvanathapuram. Cytokinesis block micronuclei assay was performed in all the samples and frequency of micronuclei was estimated. Results: Results showed a significant increase in the number of micronuclei among tobacco users when compared to that of non tobacco users. Conclusion: Estimation of frequency of micronuclei by CBMN assay can be used to assess the genotoxicity present in blood and helps in identifying tobacco users who are at a high risk for development of cancer.
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